DESIGN AND CHARACTERIZATION OF A DESK-TOP LASER PLASMA X-RAY SOURCE FOR RADIOBIOLOGY STUDIES
Daniel Adjei, Anna Wiechec, Przemyslaw Wachulak, Mesfin Getachew Ayele, Janusz Lekki, Wojciech M. Kwiatek, Andrzej Bartnik, Ladislav Pina, Henryk Fiedorowicz
Received: 5 MAY 2015,
Received revised: 22 MAY 2015,
Accepted: 29 MAY 2015,
Published Online: 18 OCT 2016
Full Text (PDF)
A compact desk-top laser-produced plasma source of soft X-rays for radiobiology research is presented. The source is based on a double-stream gas puff target and delivers nanosecond pulses of soft X-rays in the “water window” spectral range at a fluence of about 4.23´103 photon/µm2 per pulse on a sample placed inside the vacuum source chamber and about 2.60´102 photon/µm2 per pulse on a wet sample located outside the chamber in the He- environment. The source has been used to irradiate pBR322 plasmid DNA both in vacuum and the He-environment conditions. Single and double strand breaks were quantified by gel electrophoresis. The number of strand breaks increased with the increasing dose of the “water window” soft X-rays. The strand breaks of plasmid solution irradiated in helium condition may be associated with damage from water-free radicals.
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