Volume 1, Issue 2 (October 2016)

Original research papers

Biomedical Engineering


Daniel Adjei, Anna Wiechec, Przemyslaw Wachulak, Mesfin Getachew Ayele, Janusz Lekki, Wojciech M. Kwiatek, Andrzej Bartnik, Ladislav Pina, Henryk Fiedorowicz

Pages: 88-94

DOI: 10.21175/RadJ.2016.02.016

Received: 5 MAY 2015, Received revised: 22 MAY 2015, Accepted: 29 MAY 2015, Published Online: 18 OCT 2016

A compact desk-top laser-produced plasma source of soft X-rays for radiobiology research is presented. The source is based on a double-stream gas puff target and delivers nanosecond pulses of soft X-rays in the “water window” spectral range at a fluence of about 4.23´103 photon/µm2 per pulse on a sample placed inside the vacuum source chamber and about 2.60´102 photon/µm2 per pulse on a wet sample located outside the chamber in the He- environment. The source has been used to irradiate pBR322 plasmid DNA both in vacuum and the He-environment conditions. Single and double strand breaks were quantified by gel electrophoresis. The number of strand breaks increased with the increasing dose of the “water window” soft X-rays. The strand breaks of plasmid solution irradiated in helium condition may be associated with damage from water-free radicals.
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