THE ACCUMULATION PROCESS of 137Cs AND 90Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE
E.D. Marčiulionienė, O. Jefanova, V. Sakalauskas, O. Sevriukova
Received: 24 MAR 2015,
Received revised: 22 MAY 2015,
Accepted: 29 MAY 2015,
Published online: 18 OCT 2016
Full Text (PDF)
In the present study, we investigated the accumulation of 137Cs and 90Sr in compartments of the Nitellopsis obtusa cells. The effect of Sr2+, Cs+ and Ca2+, K+, which are chemical analogues of 90Sr and 137Cs, to the bioelectric parameters of these algae were studied simultaneously. The aim of this work was studying the penetration of 137Cs and 90Sr through regulating membrane barriers in the cells of starry stonewort (Nitellopsis obtusa). 137Cs and 90Sr are accumulated mainly in the cell membrane (75% to 92%) of these algae. The cell membrane as a cation exchanger regulates ion flow through the first cells diffusion barrier – its thick outer cytomembrane (the complex consisting of the cell wall and plasmalemma). Significantly, smaller amounts of 137Cs and 90Sr enter into the cytoplasm than in the cytomembrane, 10-20% and 3-10%, respectively. Analysis of the accumulation levels of 137Cs and 90Sr in the compartments of the Nitellopsis obtusa cells show their accumulation in the cell membrane as well as their active transport through outer and inner cytoplasmic membranes. Membrane potentials determined mainly by the gradient of the K+ ions are doing an important regulatory function in this process. From the obtained data it results that cells of Nitellopsis obtusa algae can be a convenient radioecological model for the study of the accumulation of radionuclides in plants at the cellular level.
- E. Epstein ”Mechanisms of ion transport through plant cell membraner”, Int. Rev. Cytol., vol. 34, pp 123-168, 1973.
- R. Nagai and U. Kishimoto “Cell wall potencial in Nitella”, Plant cell physcol., vol. 3, pp 323-334, 1964.
- M. Demarty, C. Morwan, and M. Thellien 1978. “Exchange properties of isolated cell walls of Lemna mino L.”, Plant Physcol., vol. 62(5), pp 477-481, 1978.
- L. N. Vorobyov and G.A. Kurella “The part of cell membranes in selective accumulation of ions by cells Netelle mucronata”, Biophys., vol. 10(5), pp 788-793, 1965. [in Russian]
- A. I. Leonov “The valence of the cerium in synthetic and natural aluminate and cerium silicates”, Bull. Of Phys. Sciences of the USSR. Series Chemistry, vol. 1, pp 8-13, 1963. [in Russian]
- D. Marciulioniene, R. Dusauskiene-Duz, E. Moteyunene, R. Svabiene “The radiochemoecological situation in lake Drukshiai – cooler-pond of the Ignalina NPP”, Academia, Vilnius, 1992. [in Russian]
- M.A. Hampson “Uptake of radioactivity by aquatic plants and lokation in the cells”, J. Exp. Botany, vol. 18(54), pp 17-33, 1967.
- S.J. Lambert and A.J. Davy “Water quality as a threat to aquatic plants: discriminating between the effects of nitrate, phosphate, boron and heavy metals on charophytes”, The Newphytologist, vol. 189(4), pp 1051-1059, 2011.
- T. Asaeda and T. Zaman „Heavy Metal Uptake and Tolerance of Charophytes” In P. J. Gupta DK, Corpas FJ, eds. Heavy Metal Stress in Plants, Springer-Verlag, Berlin Heidelberg, pp 111–120, 2013.
- M. A. Hampson M.A. “Uptake of radioactivity by aquatic plants and location in the cells”, J. Exp. Botany. , vol. 18(54), pp 17-33, 1967.
- D. D. Ryndina and A. Ya. Sasenko А.Я. “Methods of experimental study of accumulation of radionuclides in soils and aquatic organisms” in Marine radioecology, Naukova Dumka, Kiev, 1970. [in Russian]
- V. Schulz and F. Wicker “Radiological methods“, Mir, Moscow, 1985. [in Russian]
- E. B. Motejuniene, I. Trainauskaite, and L.N. Vorobyov “Electrophysiological properties of some species of Charophyta”, Botanical journal, vol. 60, pp 1011-1016, 1975. [in Russian]
- V. Kisnierienė, V. Sakalauskas, A. Pleskačiauskas, V. Yurin, and O. Rukšėnas “ The combined effect of Cd2+ and ACh on action potentials of Nitellopsis obtusa cells”, Central European Journal of Biology, vol. 4(3), pp 343–350, 2009.
- O. Sevriukova et al., “Charophyte Elektrogenesis as a Biomarker for Assessing the Risk from Low-Dose Ionizing Radiation to a single Plant Cell”, J. of Environ. Radioactiv., vol. 136, pp 10-15, 2014.
- S. Y. Lee et al., “Photosynthetic biomineralization of radioactive Sr via microalgal CO2 absorption”, Bioresource Technology, vol. 172, pp 449-452, 2014.
- T. Asaeda, M. D. H. J. Senavirathna, Y. Kaneko, and M. H. Rashid “Effect of calcium and magnesium on the growth and calcite encrustation of Chara fibrosa”, Aquat. Bot., vol. 113(0), pp 100-106, 2014.
- K. Siong and T. Asaeda “Calcite encrustation in macro-algae Chara and its implication to the formation of carbonate-bound cadmium”, J. Hazard. Mater., vol. 167(13), pp 1237-1241, 2009.
- Yu. A. Chitrov and L.N. Vorobyov “Determination of the activity of potassium ions in the cytoplasm and vacuoles of cells of Nitella K+ by new type sensitive microelectrode”, Plant Physiology, vol. 18(6), pp 1169-1173, 1971. [in Russian]
- N. Higinbotham “Electropotencials of plant cells”, Ann. Rev. Plant Physcol., vol. 24(2), pp 25-46, 1973.
- M. J. Beilby and M.T. Casanova “The Physiology of Characean Cells”, Springer Berlin Heidelberg, Berlin, Heidelberg, 2014.
- L.N. Vorobyov “Electrochemical parameters of plant cells as integrated physiological indicators of sustainability”, Environmental Physiology and Biogeocenology, MSU, Moscow, pp 5-26, 1979. [in Russian]
- E. Rosick, K. Stadlander, and J. Ahlers “Environmental chemicals on biomembranes”, Chemosphere, vol. 14(5), pp 529-544, 1985.
- O. Sevriukova et al., “Modifying action of tritium on the charophytes bioelectrical response to anthropogenic pollution”, Trace Elements and Electrolytes, vol. 31(2), pp 60-66, 2014.