Volume 2, Issue 1

Original research papers

Radiobiology

X-RAY EFFECTS ON STRESS RESPONSE OF THE RI-TRANSFORMANTS IN VITRO DIGITALIS PURPUREA L

Ljudmila Lioshina, Olga Bulko, Sergii Litvinov, Svitlana Pchelovska, Darina Sokolova, Anastasiya Berestyanaya, Ludmila Tonkal, Anastasia Salivon

Pages: 1-4

DOI: 10.21175/RadJ.2017.01.001

Received: 16 MAR 2016, Received revised: 16 MAY 2016, Accepted: 19 MAY 2016, Published online: 20 APR 2017

Digitalis purpurea L. is the source of a number of biologically active substances used for medical purposes. Agrobacterium rhizogenes-transformed plants show changes in the secondary metabolism. The influence of acute exposure to ionizing radiation on the modification of the pigment system and accumulation of flavonoids in Ri-transformed regenerated plants in vitro was studied. A week after the exposure to the stressor, changes in the number of chlorophylls, carotenoids and flavonoids were observed. A month later, the amount of carotenoids and the ratio of chlorophyll a/b decreased, which indicated a decrease in the photosynthetic capacity of the irradiated plants. The synthesis of flavonoids was reduced as well. But in the second passage, we saw an increase in all the parameters studied in relation to the non-irradiated samples. It showed the development of a long-acting adaptive response of plants. A nonlinear dose dependence of acute X-ray irradiation influence on in vitro D. purpurea was shown. The transformed plants were found to be more resistant to the damaging effects of radiation. We reasoned that the exposure to ionizing radiation in a certain dose interval could be used to stimulate the accumulation of biologically active substances of in vitro D. purpurea.
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