Volume 2, Issue 2

Short note



Ruslan Vazirov, Sergei Sokovnin, Maria Ulitko

Pages: 139-141

DOI: 10.21175/RadJ.2017.02.029

Received: 9 FEB 2017, Received revised: 17 APR 2017, Accepted: 22 MAY 2017, Published online: 28 OCT 2017

Postradiation complications that appeared after radiotherapy are considered the most serious problem in the treatment of various types of cancer diseases. Therefore, the research of radiomodification properties of nanoparticles is highly relevant. The aim was to study the biological activity of cerium oxide nanoparticles (CONP). CONPs were obtained by pulsed electron beam evaporation in the low gas pressure on installation NANOBIM-2. A feature of this method is the preparation of nanoparticles with a large number of structural defects of various kinds, which gives them unique physical properties, and allows expecting a high biological activity. A solution of CONPs was introduced into a normal breeding medium of the cell cultures in Petri dish to a concentration of 50 - 500 mcg/ml. To stabilize the CONPs, we used sodium citrate. Samples were irradiated on arrangement of Xstrahl 300 with 200 keV energy of X-ray and, after three days, CONPs have been added. The samples were irradiated to the absorbed doses of 5, 7.5, 10 Gy. To control the absorbed doses, we used the DTG - 4 thermo luminescent dosimeters (LiF). The analysis of biological response was performed using the Goryaeva chamber and the MTT test. The results allow us to make preliminary conclusions that the early addition of low concentrations (up to 50 mcg / ml) of CONPs will increase the sensitivity of cancer cells line Hela to radiation. Thus, the CONPs could be universal radiomodifiers to radiotherapy and their properties require subsequent studies.
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