Volume 2, Issue 2

Original research papers

Radiobiology

THE STUDY OF BIOLOGICAL EFFECTIVENESS OF U-70 ACCELERATOR CARBON IONS USING MELANOMA B-16 CLONOGENIC ASSAY

Evgeny Beketov, Elena Isaeva, Egor Malakhov, Nadezhda Nasedkina, Sergey Koryakin, Stepan Ulyanenko, Alex Solovev, Anatoly Lychagin

Pages: 90-93

DOI: 10.21175/RadJ.2017.02.020

Received: 10 FEB 2017, Received revised: 21 APR 2017, Accepted: 22 MAY 2017, Published online: 28 OCT 2017

The study was carried out using the system of accelerators (I-100, U-1.5, U-70). Ultra-precise equipment to position biological objects was applied. The dependency of melanoma B-16 cells survival on the dose of 12C ion irradiation was obtained. The carbon beam was studied within three main ranges: Bragg peak, areas before and after the peak. Dose dependence in the peak and in the area before the peak had a distinct linear pattern. In the distal part of the Bragg peak, linear-quadratic dependence was observed. Carbon ions RBE were 4.5, 1.7 and 2.4 for the peak, the areas before and after the peak, respectively.
  1. D. Habermehl et al., “The relative biological effectiveness for carbon and oxygen ion beams using the raster-scanning technique in hepatocellular carcinoma cell lines,” PLoS One, vol. 9, no. 12, p. e113591, Dec. 2014.
    DOI: 10.1371/journal.pone.0113591
    PMid: 25460352
    PMCid: PMC4252049
  2. H. Tsujii et al., “Clinical advantages of carbon-ion radiotherapy,” New Journal of Physics, vol. 10, no. 7, pp. 075009-1 – 075009-16, Jul. 2008.
    DOI: 10.1088/1367-2630/10/7/075009
  3. М. М. Кац, “Сравнение биологического действия пучков протонов и ионов 12C при лучевой терапии,” Медицинская физика, т. 2014, № 4, c. 22-25, 2014. (M. M. Kats, “Comparison of Biological impact of proton and 12C ion beams in radiation treatement,” Medical Physics, vol. 2014, no. 4, pp. 22-25, 2014.)
    Retrieved from: http://medphys.amphr.ru/_private/DB/64_22.pdf
    Retrieved on: Jan. 19, 2017.
  4. Н. В. Марков, “Дозиметрия импульсных пучков тяжелых ионов для радиобиологических исследований на ускорительном комплексе ИТЭФ–ТВН,” Дис. к.ф.-м.н., НИЦ «Курчатовский институт» ФГБУ «ГНЦ РФ ИТЭФ», Российская Федерация, 2014. (N. V. Markov, “Dosimetry of pulse beams of heavy ions for radiobiology researches at the ITEP-TVN accelerator complex,” Ph.D. Dissertation, Institute for Theoretical and Experimental Physics, Russia, 2014.)
    Retrieved from: http://www.itep.ru/science/dis/candidate_dissertation/detail.php?ID=223
    Retrieved on: Feb. 8, 2017
  5. T. Kanai et al., “Biophysical characteristics of HIMAC clinical irradiation system for heavy-ion radiation therapy,” International Journal of Radiation Oncology, Biology, Physics, vol. 44, no. 1, pp. 201-210, Apr. 1999.
    DOI: 10.1016/S0360-3016(98)00544-6
  6. K. Oonishi et al., “Different effects of carbon ion beams and X-rays on clonogenic survival and DNA repair in human pancreatic cancer stem-like cells,” Radiotherapy and Oncology, vol. 105, no. 2, pp. 258-265, Nov. 2012.
    DOI: 10.1016/j.radonc.2012.08.009
    PMid: 23017870
  7. H. Tsujii et al. “Clinical results of carbon ion radiotherapy at NIRS,” Journal of Radiation Research, vol. 48, pp. A1-A13, Mar. 2007.
    DOI: 10.1269/jrr.48.A1
  8. S. Bentzen et al., “Does variation in the in vitro cellular radiosensitivity explain the shallow clinical dose-control curve for malignant melanoma?” Int. J. Radiat. Biol., vol. 57, no. 1, pp. 117-126, 1990.
    DOI: 10.1080/09553009014550391
    PMid: 1967283
  9. R. Hill et al., “The lung-colony assay: extension to the Lewis lung tumour and the B16 melanoma--radiosensitivity of B16 melanoma cells,” Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med., vol. 27 no. 4, pp. 377-387, Mar. 1975.
    DOI: 10.1080/09553007514550351
    PMid: 1095507
  10. K. Guda et al., “An improved method for staining cell colonies in clonogenic assays,” Cytotechnology, vol. 54, no. 2, pp. 85-88, Jun. 2007.
    DOI: 10.1007/s10616-007-9083-2
    PMid: 19003022
    PMCid: PMC2267497
  11. Y. M. Antipov et al., “Transversally-flat dose field formation and primary radiobiological exercises with the carbon beam extracted from the U-70 synchrotron,” Instruments and Experimental Techniques, vol. 58, no. 4, pp. 552-561, Jul. 2015.
    DOI: 10.1134/S0020441215040016
  12. R Core Team, R Foundation for Statistical Computing, Vienna, Austria, 2016, R: A Language and Environment for Statistical Computing ver. 3.2.3.
    Retrieved from: https://cran.r-project.org/src/base/R-3/
    Retrieved on: Feb. 8, 2017.
  13. E. E. Бекетов и соавт., “Зависимость эффективности одновременного воздействия гамма-квантов и нейтронов с энергией 14 МэВ от вклада плотноионизирующего компонента,” Радиация и риск, т. 21, № 3, с. 82-90, 2012. (E. E. Beketov et al., “Dependence of simultaneous exposure of gamma-rays and neutrons with energy 14 MeV effectiveness on high-LET component contribution,” Radiation and Risk, vol. 21, no. 3, pp. 82-90, 2012.)
  14. E. V. Isaeva et al., “A comparative study of the biological effectiveness of 14-MeV neutron pulse and continuous radiation using mouse melanoma B-16 cells,” Radiation Protection Dosimetry, vol. 161, no. 1-4, pp. 478–482, Oct. 2014.
    DOI: 10.1093/rpd/nct247
    PMid: 24101654